Italian Researchers Identify New SARS-CoV-2 Gene Variants That Provide Clues to Coronavirus's Epidemiology

comunicati

Italian Researchers Identify New SARS-CoV-2 Gene Variants That Provide Clues to Coronavirus's Epidemiology

25.03.2020 - 10:45

0

Scientists say early data, generated with new NGS research panel from Thermo Fisher Scientific, suggest coronavirus is genetically stable, and could increase the effectiveness of vaccines in development

CARLSBAD, California, March 25, 2020 /PRNewswire/ -- Two teams of infectious disease researchers in Italy say they have further analyzed the SARS-CoV-2 genome from samples acquired locally to generate early data that reveal a level of genetic variability suggesting the rapidly spreading virus's genome is stable. The findings, developed using a new next-generation sequencing (NGS) research assay from Thermo Fisher Scientific, increase the likelihood that future coronavirus vaccines can have a higher rate of effectiveness and could help the global scientific community's effort to better understand the epidemiology and spread of COVID-19.

The two independent research teams from "Lazzaro Spallanzani" National Institute for Infectious Diseases (IRCCS) in Rome and the Forensic Division of the Department of Biomedical Sciences and Public Health (DSBSP) at Ancona University Hospital sequenced multiple samples and identified the presence of gene variants when compared against the original Wuhan coronavirus reference genome. Viruses that mutate rapidly over short periods of time make it challenging to develop effective vaccines that protect people against infection. The low number of variants discovered in the Italian samples two months after the virus was first sequenced in China suggests that SARS-CoV-2, which has infected more than an estimated 64,000 people in Italy and 380,000 globally, is a relatively slow-mutating pathogen. Both teams in Italy carried out the sequencing work with Thermo Fisher's new Ion AmpliSeq SARS-COV-2 Research Panel, which features a 24-hour, end-to-end workflow.

"The ability to very quickly run multiple samples and accurately decipher key changes in the virus's genetic code will be crucial for the global scientific community to stay ahead of SARS-CoV-2 and to develop strategies against it that, ultimately, can be leveraged to help resolve the pandemic," said Dr. Maria Rosaria Capobianchi, head of the Virology Department, Lazzaro Spallanzani National Institute for Infectious Diseases, which was the first research center in Europe to generated whole genome sequencing data of the coronavirus on Thermo Fisher's Ion Torrent NGS platform. "Viral genomes are dynamic and these preliminary data need further analysis to determine the biological significance of the gene variants and to investigate the evolutionary path of the coronavirus."

Professor Stefano Menzo, head of Virology at Ancona University Hospital, said: "Had we investigated other viruses we might have expected up to dozens of new mutations after so many infectious cycles in patients. Our initial data show that this is a very stable RNA virus, with only five novel variants. A virus with a stable genome is good news for vaccine development because it indicates that the effectiveness of vaccines could be more consistent, possibly over many years."

The scientists now plan to further analyze the data with Thermo Fisher's new Ion SARS-CoV-2 analysis solution* for variant annotation and consensus sequencing assembly to better understand the impact on disease severity, mode of transmission, and phylogenetic studies. The Ion AmpliSeq SARS-COV-2 Research Panel is a targeted NGS solution that analyzes the entire SARS-CoV-2 genome. It provides an efficient, high-throughput end-to-end workflow for monitoring genomic evolution, which is critical during a rapidly developing pandemic. The panel is optimized to run on the Ion GeneStudio S5 Systems.*

Panel Optimization for Genexus System Underway To further expedite NGS analysis of SARS-CoV-2 and to help meet growing customer demand, Thermo Fisher has begun to optimize the Ion AmpliSeq SARS-COV-2 Research Panel for the Ion Torrent Genexus System.* Launched in November 2019, the company's newest sequencing platform automates the entire targeted NGS workflow and can deliver specimen to report economically in as little as 14 hours. Optimization and validation of the research panel on the Genexus System is now underway in collaboration with Thermo Fisher customers.

"Thermo Fisher's immediate response to first develop a PCR SARS-CoV-2 diagnostic** assay and now a next-generation sequencing research solution to help customers investigate coronavirus is at the core of our mission," said Peter Silvester, senior vice president and president of Life Sciences Solutions at Thermo Fisher Scientific. "We share the global community's heightened concern during this unprecedented public health crisis and, for this reason, we are continuing to maximize our efforts to provide our laboratory partners, infectious disease researchers and vaccine developers with the most advanced tools in support of their very important work."

About Thermo Fisher Scientific Thermo Fisher Scientific Inc. is the world leader in serving science, with annual revenue exceeding $25 billion. Our Mission is to enable our customers to make the world healthier, cleaner and safer. Whether our customers are accelerating life sciences research, solving complex analytical challenges, improving patient diagnostics and therapies or increasing productivity in their laboratories, we are here to support them. Our global team of more than 75,000 colleagues delivers an unrivaled combination of innovative technologies, purchasing convenience and pharmaceutical services through our industry-leading brands, including Thermo Scientific, Applied Biosystems, Invitrogen, Fisher Scientific, Unity Lab Services and Patheon. For more information, please visit www.thermofisher.com.

* For Research Use Only.  Not for use in diagnostic procedures.

** Designation as an IVD or as an IVD for emergency use / special access varies from region to region.

Mauricio MinottaThermo Fisher Scientific+1 760 929 2456+1 760 805-5266 (mobile)Mauricio.minotta@thermofisher.com

Jen HeadyGreenough Communications+1 617 275 6547jheady@greenough.biz

Condividi le tue opinioni su Corriere dell'Umbria

Caratteri rimanenti: 400

Più letti oggi

Mediagallery

In motorino sul Grande Raccordo Anulare di Roma deserto. Il viaggio è surreale

In motorino sul Grande Raccordo Anulare di Roma deserto. Il viaggio è surreale

(Agenzia Vista) Roma, 02 aprile 2020 In motorino sul Grande Raccordo Anulare di Roma. Il viaggio è surreale Con i decreti emanati per l’emergenza coronavirus, il Grande Raccordo Anulare di Roma è deserto. Pochissime macchine e nessuna coda. Il viaggio in scooter è surreale Fonte: Agenzia Vista / Alexander Jakhnagiev

 
Trump: "Stanziati 350 miliardi di dollari a sostegno delle impese"

Trump: "Stanziati 350 miliardi di dollari a sostegno delle impese"

(Agenzia Vista) Washington, 02 aprile 2020 Trump stanziati 350 miliardi di dollari a sostegno delle piccole impese Le parole del Presidente degli Stati Uniti Donald Trump nel corso di una conferenza stampa alla Casa Bianca. / White House Fonte: Agenzia Vista / Alexander Jakhnagiev

 
Video Gianluca Vacchi e il suo cane, un rapporto speciale. Olaf è buono anche quando è ora di tagliare il pelo

Social

Video Gianluca Vacchi e il suo cane, un rapporto speciale. Olaf è buono anche quando è ora di tagliare il pelo

Non è un mistero che il rapporto tra Gianluca Vacchi e il suo cane sia molto stretto. Olaf El Gordo, questo il nome del suo amico a quattro zampe, è sempre presente nella ...

02.04.2020

La quarantena di Samanta Togni e Mario Russo a ritmo di musica: altra lezione di ballo. Ritmo anti Coronavirus

Social

La quarantena di Samanta Togni e Mario Russo a ritmo di musica: lezione di ballo

"La cosa buona è che le mosche le abbiamo ammazzate tutte". Scherza Samanta Togni, ballerina e star di Ballando con le stelle, fresca sposa. La sua quarantena da Coronavirus, ...

02.04.2020

Quarantena da Coronavirus, l'allenamento di Taylor Mega finisce su Instagram: "Siete pronte ragazze?"

Social

Quarantena da Coronavirus, l'allenamento di Taylor Mega finisce su Instagram: "Siete pronte ragazze?"

"Buongiorno ragazze, siete tutte pronte per un altro allenamento con Taylor Mega?". Anche la modella e showgirl pubblica sul profilo Instagram le sue intense sedute di ...

02.04.2020